Cloning and Characterisation of MEK1, an Arabidopsis Gene Encoding a Homologue of MAP Kinase Kinase

Plant Mol Biol. 1997 Dec;35(6):1057-64. doi: 10.1023/a:1005963222768.

Abstract

We report the cloning of a cDNA for MEK1, an Arabidopsis thaliana gene encoding a homologue of MAP kinase kinase (MEK). The predicted protein sequence shows 41% identity over 270 amino acids to vertebrate MEK proteins, and contains conserved features characteristic of MEK. Analysis of transcript levels show that expression of the gene is regulated by developmental processes (etiolation/de-etiolation) and by wounding. However in contrast to the rapid wound induction of MAP kinase transcripts in other plant species, MEK1 transcripts first accumulated 6-12 h after wounding.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics*
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Complementary / isolation & purification
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant
  • Genes, Plant*
  • Humans
  • MAP Kinase Kinase 1
  • Mitogen-Activated Protein Kinase Kinases*
  • Molecular Sequence Data
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / isolation & purification
  • Polymerase Chain Reaction
  • Protein-Serine-Threonine Kinases / chemistry
  • Protein-Serine-Threonine Kinases / genetics*
  • Protein-Serine-Threonine Kinases / isolation & purification
  • Protein-Tyrosine Kinases / chemistry
  • Protein-Tyrosine Kinases / genetics*
  • Protein-Tyrosine Kinases / isolation & purification
  • RNA, Messenger / biosynthesis
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Plant Proteins
  • RNA, Messenger
  • Protein-Tyrosine Kinases
  • Protein-Serine-Threonine Kinases
  • MAP Kinase Kinase 1
  • MAP2K1 protein, human
  • Mitogen-Activated Protein Kinase Kinases

Associated data

  • GENBANK/AF000977