Cleavage effect of oligoribonucleotides substituted at the cleavage sites with modified pyrimidine- and purine-nucleosides

Biochim Biophys Acta. 1997 Nov 20;1354(3):211-8. doi: 10.1016/s0167-4781(97)00099-7.

Abstract

The precursor of an RNA molecule from T4-infected E. coli cells (p2Spl RNA) has the capacity to cleave itself at specific positions [UpA (139-140) and CpA (170-171)], within a putative loop and stem structure. This sequence-specific cleavage requires at least a monovalent cation and non-ionic detergents. In order to determine the influence of the pyrimidine and purine bases on these sequence-specific cleavage reactions, we studied the cleavage reactions of hairpin loop RNAs substituted at the cleavage sites with modified pyrimidine- and purine-nucleosides. The cleavage was affected by the 2'-hydroxyl groups and the bases of the pyrimidines, and the 6-amino group of the purine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • Base Sequence
  • Binding Sites / genetics
  • Nucleic Acid Conformation
  • Oligoribonucleotides / chemical synthesis
  • Oligoribonucleotides / chemistry*
  • Oligoribonucleotides / metabolism
  • Purine Nucleosides / chemical synthesis
  • Purine Nucleosides / chemistry*
  • Purine Nucleosides / metabolism
  • Pyrimidine Nucleosides / chemical synthesis
  • Pyrimidine Nucleosides / chemistry*
  • Pyrimidine Nucleosides / metabolism
  • RNA, Catalytic / chemistry
  • RNA, Catalytic / genetics
  • RNA, Catalytic / metabolism

Substances

  • Oligoribonucleotides
  • Purine Nucleosides
  • Pyrimidine Nucleosides
  • RNA, Catalytic