Biotin synthesis in higher plants: purification and characterization of bioB gene product equivalent from Arabidopsis thaliana overexpressed in Escherichia coli and its subcellular localization in pea leaf cells

FEBS Lett. 1997 Dec 15;419(2-3):206-10. doi: 10.1016/s0014-5793(97)01458-0.

Abstract

Biotin synthase catalyses the final step in the biotin biosynthetic pathway and is encoded by the bioB gene in Escherichia coli. To investigate the conversion of dethiobiotin to biotin in the plant kingdom, the cDNA encoding the bioB gene product equivalent from Arabidopsis thaliana was used to construct an E. coli overexpression strain. The purified A. thaliana bioB gene product is a homodimer (100 kDa) with a reddish color and has an absorbance spectrum characteristic of protein with [2Fe-2S] clusters. Its intracellular compartmentation in pea leaves discloses a unique polypeptide of 39 kDa within the matrix of mitochondria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Biotin / biosynthesis*
  • Escherichia coli / genetics
  • Escherichia coli Proteins*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sulfurtransferases*

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • Recombinant Proteins
  • Biotin
  • Sulfurtransferases
  • biotin synthase, E coli