Protein-protein interaction between the RVS161 and RVS167 gene products of Saccharomyces cerevisiae

Biochim Biophys Acta. 1997 Dec 5;1343(2):187-92. doi: 10.1016/s0167-4838(97)00108-8.

Abstract

As previous studies indicated that the RVS161 and RVS167 gene products of Saccharomyces cerevisiae seem to be involved in the same cellular function, we considered the possibility of a complex between the proteins encoded by these two genes. Using the two hybrid system, we have shown that Rvs167p interacts with Rvs161p, through its N-terminal domain which contains predicted coiled-coil structures. Moreover, if a tagged Rvs 167p protein was immuno adsorbed under non-denaturing conditions, it brought along the Rvs161p protein. These results confirmed the hypothesis of an in vivo complex between the two proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cytoskeletal Proteins*
  • Dimerization
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Genes, Reporter / genetics
  • Microfilament Proteins
  • Plasmids / genetics
  • Precipitin Tests
  • Protein Conformation
  • Protein Structure, Secondary
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Cytoskeletal Proteins
  • Fungal Proteins
  • Microfilament Proteins
  • RVS161 protein, S cerevisiae
  • RVS167 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • beta-Galactosidase