Telomerase activity in human leukemic cell lines is inhibited by antisense pentadecadeoxynucleotides targeted against c-myc mRNA

Biochem Biophys Res Commun. 1997 Dec 29;241(3):775-81. doi: 10.1006/bbrc.1997.7806.

Abstract

Telomerase is a ribonucleoprotein complex that is thought to add telomeric repeats to the ends of chromosomes during the replicative phase of the cell cycle. We tested the hypothesis that proto-oncogene c-myc plays an essential role in the regulation of telomerase activity in vertebrate cells by exposing three human leukemic cell lines, HL60, U937, and K562, to 15-mer antisense c-myc oligonucleotides. All the treated cells showed a profound decrease in telomerase activity after c-myc antisense oligomer treatment, whereas cells treated with c-myc sense oligomers showed essentially no change in telomerase activity.

MeSH terms

  • Cell Line, Transformed
  • Enzyme Activation / drug effects
  • HL-60 Cells
  • Humans
  • Leukemia, Erythroblastic, Acute / enzymology
  • Leukemia, Erythroblastic, Acute / genetics
  • Oligodeoxyribonucleotides / pharmacology*
  • Oligonucleotides, Antisense / pharmacology*
  • Proto-Oncogene Proteins c-myc / genetics*
  • RNA, Messenger / genetics*
  • Telomerase / antagonists & inhibitors*
  • Telomerase / metabolism
  • Transforming Growth Factor beta / pharmacology
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Oligodeoxyribonucleotides
  • Oligonucleotides, Antisense
  • Proto-Oncogene Proteins c-myc
  • RNA, Messenger
  • Transforming Growth Factor beta
  • Tumor Necrosis Factor-alpha
  • Telomerase