Campylobacter jejuni major outer membrane protein and a 59-kDa protein are involved in binding to fibronectin and INT 407 cell membranes

FEMS Microbiol Lett. 1997 Dec 15;157(2):233-8. doi: 10.1111/j.1574-6968.1997.tb12778.x.

Abstract

Campylobacter jejuni is one of the major causes of human diarrhea throughout the world. Attachment to host cells and extracellular matrix proteins is considered to be an essential primary event in the pathogenesis of enteritis. Outer membrane proteins of three C. jejuni strains, one of which was aflagellate, were investigated for their contribution to the process of adhesion to INT 407 cell membranes and the extracellular matrix protein fibronectin. Using a ligand-binding immunoblotting assay the flagellin, the major outer membrane protein and a 59-kDa protein were detected to be involved in adhesion to both substrates. The MOMP was able to inhibit the attachment of the bacteria to INT 407 cell membranes partly, when the protein was isolated under native conditions. However, it was totally lost when the protein was isolated in the presence of SDS. The 59-kDa protein of one strain was identified by N-terminal sequencing, and regarding the first 14 amino acids it was found to be identical to the 37-kDa CadF protein just recently described as fibronectin-binding protein of C. jejuni. Especially for the aflagellate strain this protein may be of special importance for adhesion of the bacteria to different substrates.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Adhesion / drug effects
  • Bacterial Adhesion / physiology
  • Bacterial Proteins*
  • Campylobacter jejuni / physiology*
  • Cell Line
  • Cell Membrane / metabolism*
  • Cell Membrane / microbiology
  • Cytoskeletal Proteins / physiology*
  • Dystrophin-Associated Proteins
  • Fibronectins / metabolism*
  • Flagella / chemistry
  • Flagella / physiology
  • Humans
  • Immunoblotting
  • Intestinal Mucosa / cytology
  • Membrane Proteins / physiology*
  • Molecular Sequence Data
  • Porins / isolation & purification
  • Porins / metabolism*
  • Porins / pharmacology
  • Rabbits
  • Sequence Homology, Amino Acid

Substances

  • Bacterial Proteins
  • Cytoskeletal Proteins
  • Dystrophin-Associated Proteins
  • Fibronectins
  • Membrane Proteins
  • Porins
  • major outer membrane protein, Campylobacter jejuni
  • syntrophin