Abstract
Human HeLa nuclei injected into Xenopus oocytes synthesise RNA continuously for up to 1 month, and some of this RNA is translated into HeLa proteins. Nuclear proteins extracted from other cells enter the injected nuclei. This living cell system can be used for the study of transcriptional controls.
MeSH terms
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Amanitins / pharmacology
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Animals
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Biological Transport
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Cell Nucleus / metabolism*
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Female
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HeLa Cells*
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Models, Biological
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Nuclear Transfer Techniques
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Oocytes*
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Ovum*
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Protein Biosynthesis
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Proteins / metabolism
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RNA / biosynthesis*
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RNA / metabolism
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Time Factors
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Transcription, Genetic* / drug effects
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Transplantation, Heterologous
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Xenopus