Establishment and characterization of a canine T-lymphoblastoid cell line derived from malignant lymphoma

Vet Immunol Immunopathol. 1997 Oct 6;59(1-2):11-20. doi: 10.1016/s0165-2427(97)00053-6.


A canine lymphoma cell line (CL-1) was established in culture from tumor cells found in the pleural fluid of a 7-year old female Japanese terrier with thymic form lymphoma. The CL-1 cells were positive for CD45 and MHC class II and negative for CD4, CD5, CD8, Thy-1 and B-cell specific antigen and surface immunoglobulin. The CL-1 cells had a rearranged T-cell receptor beta-chain gene and a germ-line form immunoglobulin gene, indicating that the CL-1 cells represented a monoclonally expanded population of canine alpha beta T-cell lineage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Southern / veterinary
  • Dog Diseases / immunology
  • Dog Diseases / pathology*
  • Dogs
  • Female
  • Flow Cytometry / veterinary
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / genetics
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / immunology
  • Genes, Immunoglobulin / immunology
  • Histocompatibility Antigens Class II / immunology
  • Immunophenotyping / veterinary
  • Leukocyte Common Antigens / immunology
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / immunology
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / veterinary*
  • Receptors, Antigen, T-Cell, alpha-beta / immunology
  • Thymus Neoplasms / immunology
  • Thymus Neoplasms / pathology
  • Thymus Neoplasms / veterinary*
  • Tumor Cells, Cultured


  • Histocompatibility Antigens Class II
  • Receptors, Antigen, T-Cell, alpha-beta
  • Leukocyte Common Antigens