Analysis of a form of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis

Mutat Res. 1997 Dec;387(3):147-63. doi: 10.1016/s1383-5742(97)00035-5.


8-hydroxy-2'-deoxyguanosine (8-OH-dG) was first reported in 1984 as a major form of oxidative DNA damage product by heated sugar, Fenton-type reagents and X-irradiation in vitro. 8-OH-dG has been detected in cellular DNA using an HPLC-ECD method in many laboratories. Analyses of 8-OH-dG in animal organ DNA after the administration of oxygen radical-forming chemicals will be useful for assessments of their carcinogenic risk. Its analysis in human leucocyte DNA and in urine is a new approach to the assessment of an individual's cancer risk due to oxidative stress. The increase of the 8-OH-dG level in the cellular DNA, detected by HPLC-ECD method, was supported by its immunochemical detection and its enhanced repair activity. The validity of the general use of 8-OH-dG as a marker of cellular oxidative stress is discussed.

Publication types

  • Review

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Animals
  • Biomarkers / analysis*
  • Carcinogens / toxicity
  • DNA Damage*
  • DNA Repair
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / analysis
  • Deoxyguanosine / metabolism
  • Humans
  • Neoplasms / genetics*
  • Neoplasms / metabolism
  • Oxidative Stress*
  • Reproducibility of Results


  • Biomarkers
  • Carcinogens
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine