Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 11 (1), 49-62

Curcumin Inhibits IL1 Alpha and TNF-alpha Induction of AP-1 and NF-kB DNA-binding Activity in Bone Marrow Stromal Cells

Affiliations
  • PMID: 9439980

Curcumin Inhibits IL1 Alpha and TNF-alpha Induction of AP-1 and NF-kB DNA-binding Activity in Bone Marrow Stromal Cells

Y X Xu et al. Hematopathol Mol Hematol.

Abstract

We have previously demonstrated that anti-inflammatory and antioxidant compound curcumin (diferuloyl-methane) inhibits the expression of monocyte chemoattractant protein-1 (MCP-1/JE) in bone marrow stromal cells by suppressing the transcriptional activity of the MCP-1/JE gene. Since both AP-1 (TRE) and NF-kB (kB) binding motifs are present in the promoter of MCP-1/JE gene, we examined the effect of curcumin on IL1 alpha- and TNF-alpha-induced activation of ubiquitous transcription factors AP-1 and NF-kB by electrophoretic mobility shift assay and Western blotting. IL1 alpha and TNF-alpha rapidly induced both AP-1 and NF-kB DNA binding activities in +/+(-)1.LDA11 stromal cells. However, treatment of these cells with curcumin blocked the activation of AP-1 and NF-kB by both cytokines. These data suggest that inhibition of MCP-1/JE transcription by curcumin involves blocking of AP-1 and NF-kB activation by IL1 alpha or TNF-alpha.

Similar articles

See all similar articles

Cited by 33 PubMed Central articles

See all "Cited by" articles

Publication types

Feedback