Essential roles of NF-kappaB and C/EBP in the regulation of intercellular adhesion molecule-1 after respiratory syncytial virus infection of human respiratory epithelial cell cultures

J Virol. 1998 Feb;72(2):1623-6. doi: 10.1128/JVI.72.2.1623-1626.1998.

Abstract

To determine the molecular mechanism(s) of respiratory syncytial virus (RSV)-induced intercellular adhesion molecule-1 (ICAM-1) upregulation in respiratory epithelial cells (REC; A549 cell cultures), we investigated the roles of the transcription factors NF-kappaB and C/EBP. Increases in ICAM-1 message required de novo mRNA synthesis. ICAM-1 promoter constructs (luciferase reporter gene) transfected into A549 monolayers demonstrated promoter activation following RSV infection. Activation was abolished by site-specific mutation of the NF-kappaB (-228) or C/EBP (-239) sites. These data support the critical role of the activation of NF-kappaB and C/EBP in RSV-induced ICAM-1 expression by REC.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • CCAAT-Enhancer-Binding Proteins
  • Cells, Cultured
  • DNA-Binding Proteins / metabolism*
  • Epithelial Cells / metabolism
  • Epithelial Cells / virology*
  • Humans
  • Intercellular Adhesion Molecule-1 / metabolism*
  • NF-kappa B / metabolism*
  • Nuclear Proteins / metabolism*
  • Promoter Regions, Genetic
  • Respiratory Syncytial Virus Infections / metabolism*
  • Respiratory Syncytial Viruses*
  • Respiratory System / metabolism
  • Respiratory System / virology*

Substances

  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • NF-kappa B
  • Nuclear Proteins
  • Intercellular Adhesion Molecule-1