Effect of phenobarbital on hepatic CYP1A1 and CYP1A2 in the Ahr-null mouse

Biochem Pharmacol. 1998 Jan 15;55(2):235-8. doi: 10.1016/s0006-2952(97)00476-0.


Studies have suggested that phenobarbital (PB) induces members of the CYP1A subfamily by both transcriptional and post-transcriptional mechanisms. Using the Ahr -/- mice, we examined the induction of CYP1A1 and CYP1A2 by PB. CYP1A2 mRNA and protein were induced by PB in the null mice, suggesting that CYP1A2 is regulated by PB by a mechanism independent of the aryl hydrocarbon receptor (AHR). In contrast, the regulation of CYP1A1 is highly dependent on the AHR.

MeSH terms

  • Animals
  • Cytochrome P-450 CYP1A1 / biosynthesis*
  • Cytochrome P-450 CYP1A2 / biosynthesis*
  • Enzyme Induction
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Mice, Knockout
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / enzymology*
  • Phenobarbital / pharmacology*
  • Protein Biosynthesis
  • RNA, Messenger / biosynthesis
  • Receptors, Aryl Hydrocarbon / deficiency
  • Receptors, Aryl Hydrocarbon / physiology*
  • Transcription, Genetic


  • RNA, Messenger
  • Receptors, Aryl Hydrocarbon
  • Cytochrome P-450 CYP1A1
  • Cytochrome P-450 CYP1A2
  • Phenobarbital