Parvalbumin, a cross-reactive fish allergen, contains IgE-binding epitopes sensitive to periodate treatment and Ca2+ depletion

J Allergy Clin Immunol. 1998 Jan;101(1 Pt 1):67-74. doi: 10.1016/S0091-6749(98)70195-2.


Background: Type I allergy to fish is a severe health problem in countries in which a large percentage of the population derive income from fishing.

Objective: The aim of the study was to characterize cross-reactive IgE-binding components in six different fish species (cod, tuna, salmon, perch, carp, and eel). The effect of reducing extraction conditions, periodate treatment, and depletion of Ca2+ on binding of IgE to the allergens was investigated.

Methods: Extracts were prepared under nonreducing and reducing conditions. IgE-binding components were characterized by IgE immunoblotting, and cross-reactive epitopes were studied by IgE-immunoblot inhibition experiments. To reveal calcium-sensitive or carbohydrate-containing epitopes, nitrocellulose-blotted extracts were exposed to ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) and periodate.

Results: Sera from all patients allergic to fish (n = 30) displayed IgE reactivity to parvalbumin, a 12 kd protein present in fish extracts from six different species. Reducing extraction conditions had no effect on IgE binding to parvalbumins, whereas periodate treatment and depletion of protein-bound calcium led to a substantial reduction of IgE binding. Parvalbumins from six different species contained cross-reactive IgE epitopes.

Conclusion: Parvalbumin represents a cross-reactive fish allergen. It contains IgE epitopes that are sensitive to periodate treatment and Ca2+-depletion.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / chemistry*
  • Allergens / isolation & purification
  • Animals
  • Calcium
  • Cross Reactions
  • Epitopes / chemistry
  • Epitopes / isolation & purification
  • Fishes / immunology*
  • Food Hypersensitivity / etiology
  • Food Hypersensitivity / immunology
  • Humans
  • Immunochemistry
  • Immunoglobulin E / blood
  • Parvalbumins / chemistry
  • Parvalbumins / immunology*
  • Parvalbumins / isolation & purification
  • Periodic Acid
  • Species Specificity


  • Allergens
  • Epitopes
  • Parvalbumins
  • Periodic Acid
  • Immunoglobulin E
  • metaperiodate
  • Calcium