Localization of 14-3-3 proteins in the nuclei of arabidopsis and maize

Plant J. 1997 Dec;12(6):1439-45. doi: 10.1046/j.1365-313x.1997.12061439.x.


It has been demonstrated that 14-3-3 proteins are present in the nuclei of Arabidopsis thaliana and Zea mays cells using laser scanning confocal microscopy and immunocytochemistry with monoclonal antibodies against plant 14-3-3 proteins. Confirmation of nuclear localization provides insight into the range of functions normally attributed to 14-3-3 proteins, especially since the association of 14-3-3s with transcription factors is (thus far) a phenomenon unique to plants, and since 14-3-3 proteins do not possess a recognizable nuclear targeting sequence.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 14-3-3 Proteins
  • Arabidopsis / ultrastructure*
  • Cell Nucleus / ultrastructure
  • Cells, Cultured
  • Immunohistochemistry
  • Microscopy, Confocal
  • Microscopy, Electron
  • Plant Roots
  • Proteins / analysis*
  • Protoplasts
  • Tyrosine 3-Monooxygenase*
  • Zea mays / ultrastructure*


  • 14-3-3 Proteins
  • Proteins
  • Tyrosine 3-Monooxygenase