Stimulation by Rad52 of yeast Rad51-mediated recombination

Nature. 1998 Jan 22;391(6665):404-7. doi: 10.1038/34943.


In Saccharomyces cerevisiae, the RAD51 and RAD52 genes are involved in recombination and in repair of damaged DNA. The RAD51 gene is a structural and functional homologue of the recA gene and the gene product participates in strand exchange and single-stranded-DNA-dependent ATP hydrolysis by means of nucleoprotein filament formation. The RAD52 gene is important in RAD51-mediated recombination. Binding of this protein to Rad51 suggests that they cooperate in recombination. Homologues of both Rad51 and Rad52 are conserved from yeast to humans, suggesting that the mechanisms used for pairing homologous DNA molecules during recombination may be universal in eukaryotes. Here we show that Rad52 protein stimulates Rad51 reactions and that binding to Rad51 is necessary for this stimulatory effect. We conclude that this binding is crucial in recombination and that it facilitates the formation of Rad51 nucleoprotein filaments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Adenosine Triphosphate / metabolism
  • DNA, Circular / metabolism
  • DNA, Single-Stranded / metabolism
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / physiology*
  • Protein Binding
  • Rad51 Recombinase
  • Rad52 DNA Repair and Recombination Protein
  • Rec A Recombinases / metabolism
  • Recombination, Genetic*
  • Replication Protein A
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins


  • DNA, Circular
  • DNA, Single-Stranded
  • DNA-Binding Proteins
  • RAD52 protein, S cerevisiae
  • Rad52 DNA Repair and Recombination Protein
  • Replication Protein A
  • Saccharomyces cerevisiae Proteins
  • Adenosine Triphosphate
  • RAD51 protein, S cerevisiae
  • Rad51 Recombinase
  • Rec A Recombinases
  • Adenosine Triphosphatases