In vitro suppression of HIV-1 replication by ajoene [(e)-(z)-4,5,9-trithiadodeca-1,6,11-triene-9 oxide]

Biomed Pharmacother. 1997;51(9):397-403. doi: 10.1016/s0753-3322(97)89433-4.


Studies were performed to establish whether synthetic ajoene exhibited differential inhibitory activity against human immunodeficiency virus (HIV)-1 (IIIB) and to clarify the mechanism of its antiviral effects. Our results demonstrate that ajoene protected acutely infected Molt-4 cells against HIV-1 and blocked further destruction of CD4 T-cells in vitro. Ajoene showed dose-dependent inhibition, with 50% cytotoxic concentration (CTC50%) and 50% effective inhibitory concentration (EIC50%) values of 1.88 microM and about 0.35 microM, respectively, when the test compound was added before or after HIV-1 infection and incubation carried out at 37 degrees C for 4 days. Ajoene proved relatively more active than dextran sulfate in blocking HIV-1 virus-cell attachment. The mode of anti-HIV action of ajoene can be ascribed to the inhibition of early events of viral replication, particularly virus adsorption.

MeSH terms

  • Adsorption
  • Antiviral Agents / pharmacology*
  • Culture Media
  • Disulfides / pharmacology*
  • Dose-Response Relationship, Drug
  • Drug Stability
  • HIV-1 / physiology*
  • In Vitro Techniques
  • Plant Extracts / pharmacology*
  • Sulfoxides
  • Virus Replication / drug effects*


  • Antiviral Agents
  • Culture Media
  • Disulfides
  • Plant Extracts
  • Sulfoxides
  • ajoene