Characterization of nociceptin-stimulated in situ [35S]GTPgammaS binding in comparison with opioid agonist-stimulated ones in brain regions of the mice

Neurosci Lett. 1997 Nov 21;237(2-3):113-6. doi: 10.1016/s0304-3940(97)00807-0.


We studied the characterization of receptor-mediated G protein activity by nociceptin throughout brain regions, using in situ GTPgammaS binding autoradiography. Nociceptin-stimulated GTPgammaS binding was markedly observed in amygdala, hippocampal pyramidal cell layers, temporal and entorhinal cortex, infralimbic organ, anterior olfactory nucleus, and rostral part of thalamus. These nociceptin-stimulated activities were not affected by naloxone, naltrindol nor norbinaltorphimine which completely blocked mu-, delta- or kappa-opioid agonist-stimulated GTPgammaS binding, respectively. In addition, the distribution of nociceptin-stimulated activities throughout brain regions was found to be different from such opioid receptor-mediated ones.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography
  • Brain / anatomy & histology
  • Brain / physiology
  • Brain Chemistry / drug effects*
  • Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology*
  • Male
  • Mice
  • Mice, Inbred Strains
  • Opioid Peptides / pharmacology*
  • Receptors, Opioid / agonists*
  • Sulfur Radioisotopes


  • Opioid Peptides
  • Receptors, Opioid
  • Sulfur Radioisotopes
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • nociceptin