Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia

Br J Cancer. 1998;77(1):11-4. doi: 10.1038/bjc.1998.2.

Abstract

The human melanoma cell lines MM96L, A2058 and HT144 were examined for sensitivity to ionizing radiation and UVB radiation. HT144 demonstrated a significant increase in sensitivity to ionizing and UVB radiation compared with the MM96L and A2058 cells. Sensitivity to both agents was associated with susceptibility to apoptosis. Using a protein truncation assay, a mutation for the gene for ataxia telangiectasia (ATM) was identified in HT144 cells. This was confirmed to be a homozygous mutation by subsequent sequencing of the abnormal region. Protein truncation assay of the other two cell lines showed no abnormality. The results suggest that somatic mutation of the A-T gene may be important in determining tumour radiosensitivity.

MeSH terms

  • Apoptosis
  • Ataxia Telangiectasia / genetics*
  • Ataxia Telangiectasia Mutated Proteins
  • Cell Cycle Proteins
  • Cell Survival / radiation effects
  • DNA-Binding Proteins
  • Humans
  • Melanoma / genetics*
  • Mutation*
  • Protein-Serine-Threonine Kinases*
  • Proteins / analysis
  • Proteins / genetics*
  • Tumor Cells, Cultured / radiation effects
  • Tumor Stem Cell Assay
  • Tumor Suppressor Proteins
  • Ultraviolet Rays

Substances

  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Proteins
  • Tumor Suppressor Proteins
  • ATM protein, human
  • Ataxia Telangiectasia Mutated Proteins
  • Protein-Serine-Threonine Kinases