Induction of TSC-22 by treatment with a new anti-cancer drug, vesnarinone, in a human salivary gland cancer cell

Br J Cancer. 1998;77(1):71-8. doi: 10.1038/bjc.1998.11.


We undertook the present study to clarify the molecular mechanism of the effect of a new anti-cancer drug, vesnarinone, on a human salivary gland cancer cell line, TYS. We isolated TSC-22cDNA as avesnarinone-inducible gene from a cDNA library constructed from vesnarinone-treated TYS cells. TSC-22 was originally reported as a transforming growth factor (TGF)-beta-inducible gene. The expression of TSC-22 was up-regulated within a few hours after treatment with vesnarinone and was continued for 3 days. The level of TSC-22 mRNA in TYS cells was continuously increased until the cells reached confluency. Furthermore, the induction of TSC-22 by vesnarinone was inhibited by treatment with cycloheximide. When we treated the cells with an antisense oligonucleotide against TSC-22 mRNA under quiescent conditions, the antisense oligonucleotide stimulated the growth of TYS cells; however, under growing conditions the antisense oligonucleotide did not affect cell growth. Furthermore, the antisense oligonucleotide suppressed the antiproliferative effect of vesnarinone. These results suggest that TSC-22 may be a negative growth regulator and may play an important role in the antiproliferative effect of vesnarinone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Cell Cycle / drug effects
  • Cell Division / drug effects
  • Humans
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Proteins / genetics
  • Oligonucleotides, Antisense / pharmacology
  • Pyrazines
  • Quinolines / pharmacology*
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / drug effects
  • Repressor Proteins*
  • Salivary Gland Neoplasms / genetics
  • Salivary Gland Neoplasms / metabolism*
  • Salivary Gland Neoplasms / pathology
  • Transcription Factors / biosynthesis*
  • Transcription Factors / genetics
  • Tumor Cells, Cultured / drug effects
  • Up-Regulation


  • Antineoplastic Agents
  • Neoplasm Proteins
  • Oligonucleotides, Antisense
  • Pyrazines
  • Quinolines
  • RNA, Messenger
  • Repressor Proteins
  • TSC22D1 protein, human
  • Transcription Factors
  • vesnarinone