Variability in Arabidopsis Thaliana Chromosomal high-mobility-group-1-like Proteins

Eur J Biochem. 1997 Dec 15;250(3):646-52. doi: 10.1111/j.1432-1033.1997.00646.x.

Abstract

The vertebrate high-mobility-group (HMG) protein HMG1 is an abundant non-histone protein which is considered as an architectural element in chromatin. In the monocotyledonous plant maize, four different HMG1-like proteins (HMGa, HMGc1/2, HMGd) have been identified, whereas other eukaryotes usually express only two different proteins of this type. We have examined here the HMG1-like proteins of the dicotyledonous plant Arabidopsis thaliana. The isolation and analysis of cDNAs encoding five different so far uncharacterised HMG1-like proteins (now termed HMG alpha, HMG beta1/2, HMG gamma, HMG delta) from Arabidopsis indicates that the expression of multiple HMG1-like proteins is a general feature of (higher) plants. The Arabidopsis HMG1-like proteins contain an HMG domain as a common feature, but outside this conserved DNA-binding motif the amino acid sequences are significantly different indicating that this protein family displays a greater structural variability in plants than in other eukaryotes. The five HMG1-like proteins were expressed in Escherichia coli and purified. They bind with somewhat different affinity to linear double-stranded DNA. The recognition of DNA structure is evident from their preferential interaction with DNA minicircles relative to linear DNA. Reverse-transcribed PCR suggested that the five HMG1-like genes are simultaneously expressed in Arabidopsis leaves and suspension culture cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / chemistry*
  • Cells, Cultured
  • Cellulose / metabolism
  • Chromatin / chemistry
  • Chromosomal Proteins, Non-Histone / chemistry*
  • Cloning, Molecular
  • Conserved Sequence / genetics
  • DNA, Complementary / chemistry
  • DNA, Complementary / metabolism
  • DNA-Binding Proteins / chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / genetics
  • Gene Expression Regulation, Plant / genetics
  • High Mobility Group Proteins / chemistry*
  • Molecular Sequence Data
  • Phylogeny
  • Plant Proteins / analysis
  • Plant Proteins / chemistry
  • Protein Binding
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA, Complementary
  • DNA-Binding Proteins
  • High Mobility Group Proteins
  • Plant Proteins
  • Recombinant Proteins
  • Cellulose