The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA

Science. 1998 Feb 13;279(5353):1037-41. doi: 10.1126/science.279.5353.1037.

Abstract

GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The alpha subunit contains a DNA-binding domain that is a member of the ETS family, whereas the beta subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABPalpha/beta ETS domain-ankyrin repeat heterodimer bound to DNA was determined at 2. 15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an extensive protein-protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABPalpha ETS domain binds to its core GGA DNA-recognition motif.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Ankyrins / chemistry
  • Crystallography, X-Ray
  • DNA / metabolism*
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / metabolism*
  • Dimerization
  • GA-Binding Protein Transcription Factor
  • Hydrogen Bonding
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Conformation*
  • Protein Structure, Secondary
  • Proto-Oncogene Proteins / chemistry
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-ets
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Trans-Activators / chemistry
  • Trans-Activators / metabolism
  • Transcription Factors / chemistry*
  • Transcription Factors / metabolism*

Substances

  • Ankyrins
  • DNA-Binding Proteins
  • GA-Binding Protein Transcription Factor
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-ets
  • Recombinant Proteins
  • Trans-Activators
  • Transcription Factors
  • proto-oncogene protein Spi-1
  • DNA

Associated data

  • PDB/1AWC