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Comparative Study
, 330 ( Pt 1) (Pt 1), 541-7

The Molecular Basis for UV Vision in Birds: Spectral Characteristics, cDNA Sequence and Retinal Localization of the UV-sensitive Visual Pigment of the Budgerigar (Melopsittacus Undulatus)

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Comparative Study

The Molecular Basis for UV Vision in Birds: Spectral Characteristics, cDNA Sequence and Retinal Localization of the UV-sensitive Visual Pigment of the Budgerigar (Melopsittacus Undulatus)

S E Wilkie et al. Biochem J.

Abstract

Microspectrophotometric (msp) studies have shown that the colour-vision system of many bird species is based on four pigments with absorption peaks in the red, green, blue and UV regions of the spectrum. The existence of a fourth pigment (UV) is the major difference between the trichromacy of humans and the tetrachromacy of such birds, and recent studies have shown that it may play a determining role in such diverse aspects of behaviour as mate selection and detection of food. Avian visual pigments are composed of an opsin protein covalently bound via a Schiff-base linkage to the chromophore 11-cis-retinal. Here we report the cDNA sequence of a UV opsin isolated from an avian species, Melopsittacus undulatus (budgerigar or small parakeet). This sequence has been expressed using the recombinant baculovirus system; the pigment generated from the expressed protein on addition of 11-cis-retinal yielded an absorption spectrum typical of a UV photopigment, with lambdamax 365+/-3 nm. This is the first UV opsin from an avian species to be sequenced and expressed in a heterologous system. In situ hybridization of this sequence to budgerigar retinas selectively labelled a sub-set of UV cones, representing approx. 9% of the total cone population, that are distributed in a semi-regular pattern across the entire retina.

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