Molecular cloning and localization of human syntaxin 16, a member of the syntaxin family of SNARE proteins

Biochem Biophys Res Commun. 1998 Jan 26;242(3):673-9. doi: 10.1006/bbrc.1997.8029.


We have cloned a new member of the syntaxin family of proteins, designated human syntaxin 16 (hsyn16). The open reading frame encodes a polypeptide of 307 amino acids with potential coiled-coil domains and a carboxy-terminal hydrophobic tail, which is characteristic of other members of the syntaxin family. The encoded polypeptide bears sequence homology to known syntaxin molecules. Northern blot analysis revealed a single transcript that is fairly ubiquitous, being slightly more enriched in heart and pancreas. Indirect immunofluorescence localised myc-tagged hsyn16 (myc-hsyn16) to the Golgi apparatus, colocalizing well with lens culinaris agglutinin, an established Golgi marker, as well as with other Golgi SNAREs such as GS28 and syntaxin 5. Myc-hsyn16 is redistributed to the endoplasmic reticulum upon brefeldin A treatment, indicating that it is localised to the Golgi stack. The ubiquitous expression and Golgi localization of hsy16 suggest that it is involved in a vesicular transport step within the organelle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Brefeldin A
  • Cloning, Molecular
  • Cyclopentanes / pharmacology
  • Fluorescent Antibody Technique
  • Gene Expression / genetics
  • Golgi Apparatus / chemistry
  • Humans
  • Membrane Proteins / chemistry*
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Qa-SNARE Proteins
  • RNA, Messenger / analysis
  • SNARE Proteins
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Syntaxin 16
  • Vesicular Transport Proteins*


  • Cyclopentanes
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Qa-SNARE Proteins
  • RNA, Messenger
  • SNARE Proteins
  • Syntaxin 16
  • Vesicular Transport Proteins
  • Brefeldin A

Associated data

  • GENBANK/AF038897