Human immunodeficiency virus type-1-specific immune responses induced by DNA vaccination are greatly enhanced by mannan-coated diC14-amidine

Eur J Immunol. 1997 Dec;27(12):3121-9. doi: 10.1002/eji.1830271207.


Use of mannan-coated N-t-butyl-N'-tetradecyl-3-tetradecylamino-propionamidine (diC14-amidine) as an adjuvant for a DNA vaccine encoding glycoprotein 160 of human immunodeficiency virus type-1 (HIV-1) enhanced the antigen-specific immune responses. The role of interferon-gamma (IFN-gamma) and interleukin-12 in the mechanism of adjuvant action was also evaluated. Coating of diC14-amidine with mannan significantly augmented the HIV-specific delayed-type hypersensitivity reaction induced by the immunogenic DNA. HIV-1-specific cytotoxic T lymphocyte activity was also markedly enhanced by the mannan-diC14-amidine cocktail. An immunomodulatory effect of this cocktail was inhibited by treatment with anti-IFN-gamma monoclonal antibody in vivo, which suggests that IFN-gamma plays an important role in inducing cell-mediated immunity by the DNA vaccine containing this adjuvant. The results of both antigen-specific immunoglobulin isotype analysis and cytokine measurement showed that the immunogenic DNA incorporated into mannan-coated diC14-amidine elicits Th1-biased immune responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS Vaccines*
  • Amidines / immunology*
  • Animals
  • DNA, Viral / immunology*
  • Female
  • HIV-1 / immunology*
  • Humans
  • Immunization
  • Interferon-gamma / immunology
  • Mice
  • Mice, Inbred BALB C
  • T-Lymphocytes, Cytotoxic / immunology


  • AIDS Vaccines
  • Amidines
  • DNA, Viral
  • N-t-butyl-n'-tetradecyl-3-tetradecylaminopropionamidine
  • Interferon-gamma