Review article: Pathobiology of neutrophil interactions with intestinal epithelia

Aliment Pharmacol Ther. 1997 Dec;11 Suppl 3:57-62; discussion 62-3. doi: 10.1111/j.1365-2036.1997.tb00809.x.

Abstract

Neutrophil-epithelial interactions were modelled using polarized T84 cells and ligands were identified through observations of beta2-integrin dependence in patients with chronic granulomatious disease. Interactions between neutrophils and the apical membrane of crypt cells were analysed using HPLC and an in vitro model with T84 monolayers colonized by Salmonella typhimurium was used to assess neutrophil movement across the epithelium. The decline in transepithelial resistance following movement of neutrophils across the epithelial monolayer may have been due to an interaction between neutrophils and ligand ICAM-1 in which the neutrophils move along the paracellular pathway of epithelial cells. Cell surface polarity may influence these neutrophil-epithelial interactions which influence Cl secretion. These studies revealed that only strains produced in vivo were able to induce neutrophil transmigration in the in vitro model and may be indicative of new progressive therapies for inflammatory bowel disease.

Publication types

  • Review

MeSH terms

  • Antigens, CD / metabolism
  • CD18 Antigens / metabolism
  • CD47 Antigen
  • Carrier Proteins / metabolism
  • Cell Line
  • Cell Membrane / metabolism
  • Cell Movement
  • Epithelial Cells / metabolism
  • Epithelial Cells / pathology*
  • Granulomatous Disease, Chronic / physiopathology
  • Humans
  • Inflammatory Bowel Diseases / physiopathology
  • Intercellular Adhesion Molecule-1 / metabolism
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / pathology*
  • Neutrophil Activation*
  • Neutrophils / physiology*

Substances

  • Antigens, CD
  • CD18 Antigens
  • CD47 Antigen
  • CD47 protein, human
  • Carrier Proteins
  • Intercellular Adhesion Molecule-1