Spectrum of cytokine gene expression in intestinal mucosal lesions of Crohn's disease and ulcerative colitis

Digestion. 1998;59(1):73-8. doi: 10.1159/000007470.


Background/aims: We investigated the mRNA expression of spectrum of cytokines in the colonic mucosa in inflammatory bowel disease (IBD).

Methods: The expression of cytokine gene was evaluated by using the reverse transcription-polymerase chain reaction and the radioactivity of amplified cDNA standardized by coamplified beta-actin cDNA.

Results: Crohn's disease and ulcerative colitis showed significantly increased expression of IL-1beta, IL-6, IL-8 and TNF-alpha mRNA as compared with controls (p < 0.05).

Conclusion: Proinflammatory cytokines such as IL-1beta, IL-6, IL-8 and TNF-alpha are closely involved in the immune abnormalities of inflammatory mucosal lesions in IBD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Colitis, Ulcerative / genetics
  • Colitis, Ulcerative / immunology*
  • Crohn Disease / genetics
  • Crohn Disease / immunology*
  • Cytokines / biosynthesis*
  • Cytokines / genetics
  • Cytokines / immunology
  • Female
  • Gastrointestinal Neoplasms / genetics
  • Gastrointestinal Neoplasms / immunology*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • Middle Aged
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / metabolism
  • Transcription, Genetic
  • Up-Regulation


  • Cytokines
  • RNA, Messenger
  • RNA, Neoplasm