MT1-MMP on the cell surface causes focal degradation of gelatin films

FEBS Lett. 1998 Jan 9;421(2):159-64. doi: 10.1016/s0014-5793(97)01555-x.


The membrane-type matrix metalloproteinases (MT-MMPs) are a subclass of the matrix metalloproteinase (MMP) family which uniquely possess a C-terminal transmembrane domain and are initiators of an activation cascade for progelatinase A (MMP-2). Recent studies have shown that they can also efficiently directly degrade a number of matrix macromolecules. We now show that cells expressing MT1-MMP on their cell surfaces cause subjacent proteolysis of a gelatin film and that this proteolysis is inhibited by TIMP-2 but not by TIMP-1. These data indicate that expression of MT1-MMP on the cell surface may lead to both progelatinase A activation and extracellular matrix degradation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Specificity
  • CHO Cells
  • Cell Membrane
  • Cells, Cultured
  • Cricetinae
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Gelatin / metabolism*
  • Humans
  • Immunoglobulin G / immunology
  • Matrix Metalloproteinases, Membrane-Associated
  • Metalloendopeptidases / biosynthesis
  • Metalloendopeptidases / genetics
  • Metalloendopeptidases / metabolism*
  • Sheep


  • Immunoglobulin G
  • Gelatin
  • Matrix Metalloproteinases, Membrane-Associated
  • Metalloendopeptidases