Increased replication of T-cell-tropic HIV strains and CXC-chemokine receptor-4 induction in T cells treated with macrophage inflammatory protein (MIP)-1alpha, MIP-1beta and RANTES beta-chemokines

AIDS. 1998 Jan 22;12(2):183-90. doi: 10.1097/00002030-199802000-00008.


Objective and design: To study, in T-lymphoid cells, the effects of macrophage inflammatory protein (MIP)-1alpha, MIP-1beta and RANTES beta-chemokines on the replication of T-cell-tropic HIV-1 strains, since it has been reported that beta-chemokines interfere with the replication of macrophage-tropic HIV-1 strains, but not T-cell-tropic strains.

Methods: Freshly phytohaemagglutinin (PHA)-activated peripheral blood lymphocytes (PBL) and cultured PHA-activated T cells from healthy volunteers, as well as the C8166 T-cell line, were treated overnight with beta-chemokines before infection with T-cell-tropic HIV-1 isolates, or human T-lymphotropic virus type IIIB. HIV replication was followed by detecting the production of infectious particles, p24 antigen, and viral sequences. CXC-chemokine receptor (CXCR)-4 expression was followed by detection and quantification of specific transcripts.

Results: Pretreatment of T cells with MIP-1alpha, MIP-1beta and RANTES affected T-cell-tropic strains, increased the replication of HIV-1beta and HIV-1RPdT strains dose-dependently, as well as virus absorption and provirus DNA accumulation. These findings were associated with increased accumulation of CXCR-4 transcripts, and mediated by the protein tyrosine kinase signalling. Moreover, beta-chemokines stimulated PBL proliferation.

Conclusions: Beta-chemokines increase the adsorption and replication of at least some T-cell-tropic HIV-1 strains, and this is related to stimulated expression of the CXCR-4 coreceptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division
  • Cell Line
  • Cells, Cultured
  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokine CCL5 / pharmacology*
  • Chemotaxis, Leukocyte
  • DNA, Viral / blood
  • Deltaretrovirus / immunology
  • Deltaretrovirus / physiology
  • HIV-1 / immunology
  • HIV-1 / physiology*
  • Humans
  • Lymphocyte Activation
  • Macrophage Inflammatory Proteins / pharmacology*
  • Polymerase Chain Reaction
  • Protein-Tyrosine Kinases / metabolism
  • Proviruses / isolation & purification
  • RNA-Directed DNA Polymerase
  • Receptors, CXCR4 / biosynthesis*
  • Receptors, CXCR4 / genetics
  • Signal Transduction
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / virology*
  • Virus Replication


  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokine CCL5
  • DNA, Viral
  • Macrophage Inflammatory Proteins
  • Receptors, CXCR4
  • Protein-Tyrosine Kinases
  • RNA-Directed DNA Polymerase