Isolation of specific DNA probes for detection of Cryptococcus neoformans

Mycoses. 1997 Dec;40(9-10):385-9. doi: 10.1111/j.1439-0507.1997.tb00256.x.

Abstract

We report the construction of a genomic library and isolation of repetitive DNA probes from Cr. neoformans that are species- and variety-specific. The inserts of the genomic library were in a range of 0.3-1.8 kb, which were in normal distribution in length. Among 592 clones of the genomic library, there were 400(67.6%) clones inserted repetitive DNA sequence and 192(32.4%) inserted single copy DNA sequence of the Cr. neoformans genome. From the library, three specific probes were identified. Clone pCNIIA6 was serotype A-specific, pCNIIB5 species-specific, and pCNIIIG1 variety-specific (var. neoformans). On the basis of the high specificity and sensitivity of the signals observed by hybridization, we suggest that these probes provide a means for both biotyping and early diagnosis of Cr. neoformans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cryptococcus neoformans / genetics*
  • Cryptococcus neoformans / isolation & purification*
  • DNA Probes / chemistry
  • DNA Probes / isolation & purification*
  • DNA, Fungal / isolation & purification*
  • DNA, Recombinant / chemical synthesis
  • Enzymes
  • Gene Library
  • Genetic Testing
  • Humans
  • In Situ Hybridization
  • Plasmids / chemical synthesis

Substances

  • DNA Probes
  • DNA, Fungal
  • DNA, Recombinant
  • Enzymes
  • novozym 234