Computer analysis of the entire budding yeast genome for putative targets of the GCN4 transcription factor

Curr Genet. 1998 Jan;33(1):16-20. doi: 10.1007/s002940050303.


The completion of the yeast genome project enables an analysis of various phenomena for a whole eukaryotic genome. We aimed at characterizing a full spectrum of target genes for a transcription activator, and specifically characterized putative targets for GCN4 in the budding yeast. The results suggest that about 1% of the genes are regulated by GCN4 and that these genes code for proteins involved in amino-acid and nucleotide metabolism. Our analysis proposes that, when enough data about the binding nature of a transcription factor exists, it is possible to identify its putative targets and also to try and assign a physiological role for this transcription factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites / genetics
  • Chromosome Mapping*
  • Computational Biology / methods
  • DNA-Binding Proteins*
  • Databases, Factual
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Genes, Fungal
  • Genome, Fungal*
  • Multigene Family
  • Protein Kinases / genetics*
  • Protein Kinases / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Software*
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism


  • DNA-Binding Proteins
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Protein Kinases