Induction of macrophage-inflammatory protein 1alpha (MIP-1alpha) by interferon-alpha

Exp Hematol. 1998 Feb;26(2):117-23.


The biological functions of macrophage-inflammatory protein 1alpha (MIP-1alpha) include the regulation of inflammatory processes and the inhibition of early hematopoiesis. We investigated the regulation of MIP-1alpha in various cell types in response to IFN-alpha and IL-4 by mRNA analysis and examination of MIP-1alpha protein levels in culture supernatants. In peripheral blood mononuclear cells (PBMNCs) and purified monocytes, IFN-alpha induced MIP-1alpha mRNA expression and stimulated MIP-1alpha secretion in LPS-activated monocytes to almost twice the value observed with LPS alone, whereas IL-4 caused a reduction by 80-95%. The stimulatory effect of IFN-alpha on MIP-1alpha production appeared to be dominant over its inhibition by IL-4. This effect was most pronounced after prolonged incubation periods. In fibroblasts obtained from human foreskin, IFN-alpha in combination with IL-1 stimulated MIP-1alpha secretion to more than three times the level obtained with IL-1. Stimulation of MIP-1alpha expression by IFN-alpha may indirectly influence inflammatory processes and the regulation of early hematopoiesis.

MeSH terms

  • Cells, Cultured
  • Chemokine CCL3
  • Chemokine CCL4
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Fibroblasts / immunology
  • Gene Expression Regulation / drug effects
  • Humans
  • Infant, Newborn
  • Interferon alpha-2
  • Interferon-alpha / pharmacology*
  • Interleukin-1 / pharmacology*
  • Kinetics
  • Macrophage Inflammatory Proteins / biosynthesis*
  • Male
  • Monocytes / cytology
  • Monocytes / drug effects
  • Monocytes / immunology*
  • RNA, Messenger / biosynthesis
  • Recombinant Proteins / pharmacology
  • Skin / cytology
  • Skin / drug effects
  • Skin / immunology
  • Transcription, Genetic / drug effects*


  • Chemokine CCL3
  • Chemokine CCL4
  • Interferon alpha-2
  • Interferon-alpha
  • Interleukin-1
  • Macrophage Inflammatory Proteins
  • RNA, Messenger
  • Recombinant Proteins