Flow cytometry of mitotic cells

Exp Cell Res. 1998 Feb 1;238(2):498-502. doi: 10.1006/excr.1997.3862.

Abstract

We have developed a procedure using flow cytometric measurement of a mitosis-specific antigen that may be used to count mitotic cells and sort them from non-mitotic cells. The procedure may also be used in conjunction with measurement of cellular DNA content and of bromodeoxyuridine incorporation into cellular DNA to assign cells to the G1/G0, S, G2, or M phase of the cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antibody Specificity
  • Antigens / analysis
  • Breast Neoplasms
  • Carcinoma
  • Cell Separation
  • DNA, Neoplasm / analysis
  • Flow Cytometry / methods*
  • Humans
  • Interphase
  • Mitosis*
  • Nocodazole / pharmacology
  • Phosphoproteins / analysis
  • RNA-Binding Proteins / analysis
  • Tumor Cells, Cultured
  • tau Proteins / analysis

Substances

  • Antibodies, Monoclonal
  • Antigens
  • DNA, Neoplasm
  • Phosphoproteins
  • RNA-Binding Proteins
  • nucleolin
  • tau Proteins
  • Nocodazole