Multiple protein-binding sites in the TAS-region of human and rat mitochondrial DNA

Biochem Biophys Res Commun. 1998 Feb 4;243(1):36-40. doi: 10.1006/bbrc.1997.8052.

Abstract

To study the molecular mechanisms responsible for the regulation of mitochondrial DNA copy number, in vivo and in organello dimethyl sulfate footprinting experiments in human fibroblasts and rat liver mitochondria were carried out. By this approach we identified in both species two specific protein binding sites in the 3' region of the displacement loop of mitochondrial DNA. One site contains the TAS-D element of human and rat mitochondrial DNA; the other covers TAS-C and TAS-B in human, whereas in rat it comprises part of TAS-B. We suggest that the protected sequences might be the site of action of protein factors involved in the premature termination of mitochondrial DNA heavy-strand synthesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites / genetics
  • Cattle
  • DNA Footprinting
  • DNA, Mitochondrial / chemistry
  • DNA, Mitochondrial / genetics*
  • DNA, Mitochondrial / metabolism*
  • DNA-Binding Proteins / metabolism
  • Humans
  • Molecular Sequence Data
  • Protein Binding
  • Rats
  • Species Specificity

Substances

  • DNA, Mitochondrial
  • DNA-Binding Proteins

Grant support