Inhibition of pulmonary eosinophilia in P-selectin- and ICAM-1-deficient mice

Am J Respir Cell Mol Biol. 1998 Feb;18(2):218-25. doi: 10.1165/ajrcmb.18.2.2829.


Adhesion molecule expression by pulmonary endothelial cells is considered to play an important role in the recruitment of circulating leukocytes to sites of inflammation in the lung. We have used P-selectin- and intercellular adhesion molecule type 1 (ICAM-1)-deficient mice to determine whether these adhesion molecules are important to pulmonary eosinophil recruitment after allergen challenge. There was a significant inhibition of lung tissue eosinophil recruitment in ICAM-1-deficient mice (approximately 84% inhibition compared to wild-type mice) and P-selectin-deficient mice (approximately 67% inhibition compared to wild-type mice) 3 h after allergen challenge. The number of bronchoalveolar lavage (BAL) eosinophils in P-selectin-deficient and ICAM-1-deficient mice was also significantly reduced compared with wild-type mice. Levels of BAL eosinophil peroxidase (EPO) were significantly lower in ICAM-1-deficient mice (0.21 +/- 0.03 EPO units) compared with wild-type mice (3.34 +/- 0.65 EPO units). There was no significant difference in the degree of inhibition of eosinophil recruitment in ICAM-1-deficient mice at the three time points (3, 12, and 24 h) of study after allergen challenge. However, in P-selectin-deficient mice there was a decline in the degree of inhibition of eosinophil recruitment from 3 h (67% inhibition) and 12 h (72% inhibition) postchallenge, to 24 h postchallenge (38% inhibition), suggesting that other adhesion molecules may be playing a more prominent role than P-selectin at later time points. These studies suggest an important role for ICAM-1 and P-selectin in eosinophil recruitment to the lung after allergen challenge.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens / immunology
  • Bronchoalveolar Lavage Fluid / immunology*
  • Chemotaxis, Leukocyte
  • Disease Models, Animal
  • Eosinophil Peroxidase
  • Eosinophils / enzymology
  • Female
  • Hypersensitivity, Immediate
  • Intercellular Adhesion Molecule-1 / genetics
  • Intercellular Adhesion Molecule-1 / immunology*
  • Lung / immunology
  • Mice
  • Mice, Mutant Strains
  • Ovalbumin / immunology
  • P-Selectin / genetics
  • P-Selectin / immunology*
  • Peroxidases / analysis
  • Pulmonary Eosinophilia / chemically induced
  • Pulmonary Eosinophilia / immunology*
  • Skin / immunology


  • Antigens
  • P-Selectin
  • Intercellular Adhesion Molecule-1
  • Ovalbumin
  • Eosinophil Peroxidase
  • Peroxidases