Mucosal chemokine activity in Helicobacter pylori infection

J Clin Gastroenterol. 1997;25 Suppl 1:S203-10. doi: 10.1097/00004836-199700001-00032.

Abstract

We examined secretion, mRNA expression, and histologic localization of interleukin-8 (IL-*) and growth-related gene product-alpha (GRO alpha) in the Helicobacter pylori-infected gastric antral mucosa. Antral biopsies were obtained from an area of endoscopically intact mucosa. Significantly higher levels of IL-8 and GRO alpha were secreted in organ cultures from patients with H. pylori infection, and their elevation was prominent in patients with duodenal ulcer. There was a significant association between these alpha-chemokine levels and histologic grades of activity, inflammation, and H. pylori density. In fresh antral biopsies, IL-8 and GRO alpha mRNA expression was detected more frequently in H. pylori-infected patients compared with those without infection. Immunofluorescence microscopy showed localization of IL-8 and GRO alpha proteins in gastric epithelial cells and infiltrating CD68+ macrophages. In the chemotaxis assay, a significant positive correlation was found between neutrophil migration induced by the organ culture supernatants and their contents of IL-8 and GRO alpha. After H. pylori eradication, a significant decrease was observed in IL-8 and GRO alpha levels detected in organ cultures. In conclusion, mucosal alpha-chemokine activity correlates well with histologic severity of H. pylori-associated antral gastritis and can be used to predict the effects of H. pylori eradication therapy.

MeSH terms

  • Adult
  • Biopsy
  • Chemokine CXCL1
  • Chemokines / biosynthesis*
  • Chemokines, CXC*
  • Chemotactic Factors / biosynthesis*
  • Chemotaxis, Leukocyte
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Gastric Mucosa / metabolism*
  • Gastric Mucosa / microbiology
  • Gastritis / metabolism
  • Gastritis / microbiology*
  • Gene Expression
  • Growth Substances / biosynthesis*
  • Helicobacter Infections / metabolism*
  • Helicobacter pylori*
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-8 / biosynthesis*
  • Male
  • Middle Aged
  • Neutrophils / physiology
  • Peptic Ulcer / metabolism
  • Peptic Ulcer / microbiology*
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics

Substances

  • CXCL1 protein, human
  • Chemokine CXCL1
  • Chemokines
  • Chemokines, CXC
  • Chemotactic Factors
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-8
  • RNA, Messenger