Abstract
Tyrosine phosphorylation has been implicated in growth-cone guidance through genetic, biochemical, and pharmacological studies. Adapter proteins containing src homology 2 (SH2) domains and src homology 3 (SH3) domains provide a means of linking guidance signaling through phosphotyrosine to downstream effectors regulating growth-cone motility. The Drosophila adapter, Dreadlocks (Dock), the homolog of mammalian Nck containing three N-terminal SH3 domains and a single SH2 domain, is highly specialized for growth-cone guidance. In this paper, we demonstrate that Dock can couple signals in either an SH2-dependent or an SH2-independent fashion in photoreceptor (R cell) growth cones, and that Dock displays different domain requirements in different neurons.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Animals
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Animals, Genetically Modified
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Axons / physiology*
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Axons / ultrastructure
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Cloning, Organism
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Crosses, Genetic
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Drosophila / genetics
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Drosophila / physiology
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Drosophila Proteins
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Eye / innervation
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Ganglia, Invertebrate / physiology*
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Humans
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Larva
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Mammals
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Mutagenesis, Insertional
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Nerve Fibers / physiology*
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Nerve Fibers / ultrastructure
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Nerve Tissue Proteins / biosynthesis
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / metabolism*
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Oncogene Proteins / chemistry
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Oncogene Proteins / metabolism
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Signal Transduction*
Substances
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Adaptor Proteins, Signal Transducing
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Drosophila Proteins
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Nck protein
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Nerve Tissue Proteins
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Oncogene Proteins
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Recombinant Proteins
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dock protein, Drosophila