This study evaluated the effect of multiple applications of the one-bottle adhesives Prime & Bond, One-Step, and Tenure Quik on the shear bond strength of composite to dentin. In addition, the study examined the effects of surface moisture and dual-cure composite on bond strengths of One-Step and Tenure Quik respectively. The adhesive systems were bonded to the occlusal dentin of extracted human teeth. Control group specimens received only two applications of adhesive, as recommended by the manufacturers. Two experimental groups of each system received a greater number of adhesive applications. Additional experimental groups using only two adhesive applications were made to test modifications in surface moisture and composite type for One-Step and Tenure Quik. Shear bond strengths were determined using an Instron Universal Testing Machine. The mean shear bond strength of the Prime & Bond control group was significantly greater than that of the other two control groups. Multiple applications of adhesive decreased the bond strength of each system, but the difference was significant only for four applications of One-Step. The method of moisture removal (compressed air or blotting) had no effect on shear bond strength of One-Step. The shear bond strength of Tenure Quik was not affected by the type of composite used. Prime & Bond had significantly higher shear bond strengths to dentin than either One-Step or Tenure Quik. Both Prime & Bond and One-Step had significantly higher bond strengths than Tenure Quik.