Analysis of the isopentenyl diphosphate isomerase gene family from Arabidopsis thaliana

Plant Mol Biol. 1998 Jan;36(2):323-8. doi: 10.1023/a:1005935516274.

Abstract

Two Arabidopsis thaliana cDNAs (IPP1 and IPP2) encoding isopentenyl diphosphate isomerase (IPP isomerase) were isolated by complementation of an IPP isomerase mutant strain of Saccharomyces cerevisiae. Both cDNAs encode enzymes with an amino terminus that may function as a transit peptide for localization in plastids. At least 31 amino acids from the amino terminus of the IPP1 protein and 56 amino acids from the amino terminus of the IPP2 protein are not essential for enzymatic activity. Genomic DNA blot analysis confirmed that IPP1 and IPP2 are derived from a small gene family in A. thaliana. Based on northern analysis expression of both cDNAs occurs predominantly in roots of mature A. thaliana plants grown to the pre-flowering stage.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics*
  • Base Sequence
  • Carbon-Carbon Double Bond Isomerases / biosynthesis
  • Carbon-Carbon Double Bond Isomerases / chemistry
  • Carbon-Carbon Double Bond Isomerases / genetics*
  • DNA Primers
  • Genes, Plant*
  • Hemiterpenes
  • Molecular Sequence Data
  • Multigene Family*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / isolation & purification
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • DNA Primers
  • Hemiterpenes
  • Recombinant Proteins
  • Carbon-Carbon Double Bond Isomerases
  • isopentenyldiphosphate delta-isomerase

Associated data

  • GENBANK/U47324
  • GENBANK/U49259