Molecular cloning of a novel fimbrin-like cDNA from Arabidopsis thaliana

Plant Mol Biol. 1998 Jan;36(1):23-31. doi: 10.1023/a:1005884112192.

Abstract

Fimbrin is a 68-70 kDa actin-bundling protein in animal cells and lower eukaryotes that participates in diverse morphogenetic processes by cross-linking actin filaments into bundles. Here we report the cloning by degenerate polymerase chain reaction (PCR) of ATFIM1, a 2.3 kb cDNA from Arabidopsis thaliana that codes for a novel 76 kDa fimbrin-like polypeptide (AtFim1). The predicted sequence of AtFim1 shares ca. 40% identity with nonplant fimbrins and contains two tandem repeats, each possessing a 27 amino acid region identified as a putative actin-binding domain in fimbrins and in a larger family of actin cross-linking proteins. Preceding the tandem repeats at the amino terminus of AtFim1 is a single-EF-hand-like domain with moderate homology to calmodulin-like calcium-binding proteins. AtFim1 differs from non-plant fimbrins, however, in that it contains an extended carboxy-terminal tail of ca. 65 amino acids. ATFIM1 is encoded by a single gene, although sequencing of two partial fimbrin-like expressed sequence tag (EST) clones indicates that Arabidopsis contains at least two fimbrin-like proteins. Northern blot analysis and reverse-transcription PCR (RT-PCR) demonstrated that ATFIM1 is expressed in all major organs examined (roots, leaves, stems, flowers and siliques). This is the first report of the cloning of a full length plant gene that encodes a putative actin filament-bundling protein.

MeSH terms

  • Actins / metabolism
  • Amino Acid Sequence
  • Animals
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins*
  • Base Sequence
  • Chickens
  • Cloning, Molecular
  • DNA, Complementary
  • Humans
  • Membrane Glycoproteins / chemistry*
  • Microfilament Proteins / chemistry
  • Molecular Sequence Data
  • Molecular Weight
  • Plant Proteins / biosynthesis*
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Polymerase Chain Reaction
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Saccharomyces cerevisiae / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • ATFIM1 protein, Arabidopsis
  • Actins
  • Arabidopsis Proteins
  • DNA, Complementary
  • Membrane Glycoproteins
  • Microfilament Proteins
  • Plant Proteins
  • Recombinant Proteins
  • plastin

Associated data

  • GENBANK/U66424
  • GENBANK/U77673