Prevalence and characterization of perinuclear anti-neutrophil cytoplasmic antibodies (P-ANCA) directed against HMG1 and HMG2 in ulcerative colitis (UC)

Clin Exp Immunol. 1998 Feb;111(2):402-7. doi: 10.1046/j.1365-2249.1998.00491.x.


In a previous study, we reported that the high mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 were novel target antigens of P-ANCA. In this study, we determined the immunodiagnostic value of anti-HMG1/HMG2 antibodies in patients with UC. Sixty sera from patients with UC were tested for reactivity with HMG1 and HMG2 by means of ELISA. Anti-HMG1 antibody was detected in 32% of patients (40% of P-ANCA+ patients). Anti-HMG2 antibody was detected in 33% (40% of P-ANCA+ patients). Thirty-five percent of sera were positive for antibody to either HMG1 or HMG2 (43% of P-ANCA+ patients). P-ANCA+ patients expressed anti-HMG1/HMG2 antibodies with significantly greater frequency compared with P-ANCA- patients. Furthermore, the anti-HMG1/HMG2 antibodies were significantly related to disease activity in UC. Sixteen of the 18 UC patients, who had high titres of anti-HMG1 or -HMG2 antibody during the active phase, showed lower titres in the inactive phase. Anti-HMG1/HMG2 antibodies appear to be useful as a marker for disease activity in UC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Antibodies / analysis
  • Antibodies / blood
  • Antibodies, Antineutrophil Cytoplasmic / blood
  • Antibodies, Antineutrophil Cytoplasmic / immunology*
  • Blotting, Western
  • Cathepsin G
  • Cathepsins / immunology
  • Child
  • Colitis, Ulcerative / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • High Mobility Group Proteins / immunology*
  • Humans
  • Lactoferrin / immunology
  • Male
  • Middle Aged
  • Serine Endopeptidases
  • Swine


  • Antibodies
  • Antibodies, Antineutrophil Cytoplasmic
  • High Mobility Group Proteins
  • Cathepsins
  • Lactoferrin
  • Serine Endopeptidases
  • CTSG protein, human
  • Cathepsin G