The aim of this laboratory based study was to determine the shaping ability of Lightspeed nickel-titanium rotary instruments in simulated root canals. A total of 40 canals with four different shapes in terms of angle and position of curve were prepared with Lightspeed instruments using the stepback technique recommended by the manufacturer. This report describes the efficacy of the instruments in terms of prevalence of canal aberrations, the amount and direction of canal transportation, and overall postoperative shape. Preoperative and postoperative images of the canals were taken using a video camera attached to a computer with image analysis software. The preoperative and postoperative views were superimposed to highlight the amount and position of material removed during preparation. Only one (2.5%) zip and one elbow were created, with no ledges, perforations, danger zones, or blockages being produced. At specific points along the canal length there were highly significant differences (p < 0.001) between the canal shapes in total canal width and in the amount of resin removed from the inner and outer aspects of the curve. The direction of canal transportation at the end point of preparation was most frequently toward the outer aspect of the curve; although in half of the canals, transportation was either directed toward the inner aspect or not observed. At the apex of the curve, the beginning of the curve, and halfway to the orifice, transportation was reversed with the majority of canals being transported toward the inner aspect of the curve. Mean absolute transportation was small and was below 0.06 mm at every position except the orifice. Overall, Lightspeed rotary instruments prepared canals well and would appear to be a valuable addition to the endodontic armamentarium.