Two Members of the Tcf Family Implicated in Wnt/beta-catenin Signaling During Embryogenesis in the Mouse

Mol Cell Biol. 1998 Mar;18(3):1248-56. doi: 10.1128/mcb.18.3.1248.

Abstract

Tcf transcription factors interact with beta-catenin and Armadillo to mediate Wnt/Wingless signaling. We now report the characterization of genes encoding two murine members of the Tcf family, mTcf-3 and mTcf-4. mTcf-3 mRNA is ubiquitously present in embryonic day 6.5 (E6.5) mouse embryos but gradually disappears over the next 3 to 4 days. mTcf-4 expression occurs first at E10.5 and is restricted to di- and mesencephalon and the intestinal epithelium during embryogenesis. The mTcf-3 and mTcf-4 proteins bind a canonical Tcf DNA motif and can complex with the transcriptional coactivator beta-catenin. Overexpression of Wnt-1 in a mammary epithelial cell line leads to the formation of a nuclear complex between beta-catenin and Tcf proteins and to Tcf reporter gene transcription. These data demonstrate a direct link between Wnt stimulation and beta-catenin/Tcf transcriptional activation and imply a role for mTcf-3 and -4 in early Wnt-driven developmental decisions in the mouse embryo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cell Nucleus / metabolism
  • Chickens
  • Cloning, Molecular
  • Cytoskeletal Proteins / metabolism*
  • Embryonic and Fetal Development
  • Gene Expression
  • Genes, Reporter
  • HMGB Proteins*
  • Humans
  • Luciferases / genetics
  • Mice
  • Molecular Sequence Data
  • PC12 Cells
  • Proto-Oncogene Proteins / biosynthesis
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism*
  • Rats
  • Signal Transduction*
  • TCF Transcription Factors
  • Trans-Activators*
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factor 7-Like 2 Protein
  • Transcription Factors / biosynthesis
  • Transcription Factors / metabolism*
  • Transcriptional Activation
  • Wnt Proteins
  • Wnt1 Protein
  • Zebrafish Proteins*
  • beta Catenin

Substances

  • CTNNB1 protein, human
  • CTNNB1 protein, mouse
  • Ctnnb1 protein, rat
  • Cytoskeletal Proteins
  • HMGB Proteins
  • Proto-Oncogene Proteins
  • TCF Transcription Factors
  • TCF7L1 protein, human
  • TCF7L2 protein, human
  • Tcf7l1 protein, mouse
  • Tcf7l1 protein, rat
  • Tcf7l2 protein, mouse
  • Tcf7l2 protein, rat
  • Trans-Activators
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factor 7-Like 2 Protein
  • Transcription Factors
  • WNT1 protein, human
  • Wnt Proteins
  • Wnt1 Protein
  • Wnt1 protein, mouse
  • Wnt1 protein, rat
  • Zebrafish Proteins
  • beta Catenin
  • Luciferases

Associated data

  • GENBANK/AJ223069
  • GENBANK/AJ223070