Natural ligand of mouse CD1d1: cellular glycosylphosphatidylinositol

Science. 1998 Mar 6;279(5356):1541-4. doi: 10.1126/science.279.5356.1541.


Mouse CD1d1, a member of the CD1 family of evolutionarily conserved major histocompatibility antigen-like molecules, controls the differentiation and function of a T lymphocyte subset, NK1+ natural T cells, proposed to regulate immune responses. The CD1d1 crystal structure revealed a large hydrophobic binding site occupied by a ligand of unknown chemical nature. Mass spectrometry and metabolic radiolabeling were used to identify cellular glycosylphosphatidylinositol as a major natural ligand of CD1d1. CD1d1 bound glycosylphosphatidylinositol through its phosphatidylinositol aspect with high affinity. Glycosylphosphatidylinositol or another glycolipid could be a candidate natural ligand for CD1d1-restricted T cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, CD1 / chemistry
  • Antigens, CD1 / isolation & purification
  • Antigens, CD1 / metabolism*
  • Binding Sites
  • Glycosylphosphatidylinositols / chemistry
  • Glycosylphosphatidylinositols / metabolism*
  • Ligands
  • Mass Spectrometry
  • Mice
  • Solubility
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • T-Lymphocyte Subsets / immunology


  • Antigens, CD1
  • Glycosylphosphatidylinositols
  • Ligands