A mimic as internal standard to monitor PCR analysis of food-borne pathogens

Lett Appl Microbiol. 1998 Jan;26(1):9-11. doi: 10.1046/j.1472-765x.1998.00256.x.

Abstract

This report describes a method for the construction of a mimic molecule for monitoring PCR. A blue cheese homogenate spiked with Yersinia enterocolitica was used to demonstrate how false-negative PCR results may be avoided using the mimic. Further, the possibility of estimating bacterial numbers using the mimic was illustrated by varying the target concentration.

MeSH terms

  • Bacteriological Techniques*
  • Cheese / microbiology
  • DNA, Bacterial / analysis*
  • Electrophoresis, Agar Gel
  • Ethidium
  • False Negative Reactions
  • Food Microbiology*
  • Polymerase Chain Reaction / standards*
  • Reference Standards
  • Yersinia enterocolitica / genetics*

Substances

  • DNA, Bacterial
  • Ethidium