Abstract
Amino acid transporters of the yeast plasma membrane (permeases) belong to a family of integral membrane proteins with pronounced structural similarity. We present evidence that a member of this family, encoded by the open reading frame (ORF) YDR160w (SSY1), is required for the expression of a set of transporter genes. Thus, deletion of the SSY1 gene causes loss of leucine-inducible transcription of the amino acid permease genes BAP2, TAT1 and BAP3 (ORF YDR046c) and the peptide transporter, PTR2. D-leucine can generate the signal without entering the cell. We propose that Ssy1p is situated in the plasma membrane and is involved in sensing leucine in the medium.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Transport Systems
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Amino Acids / metabolism
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Fungal Proteins / metabolism
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Gene Deletion
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Gene Expression Regulation, Fungal*
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Genes, Fungal
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Leucine / metabolism
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Membrane Transport Proteins / genetics*
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Membrane Transport Proteins / metabolism
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Signal Transduction
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Transcription, Genetic
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Transcriptional Activation
Substances
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Amino Acid Transport Systems
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Amino Acids
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Carrier Proteins
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Fungal Proteins
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Membrane Proteins
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Membrane Transport Proteins
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PTR2 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Leucine