The permease homologue Ssy1p controls the expression of amino acid and peptide transporter genes in Saccharomyces cerevisiae

Mol Microbiol. 1998 Feb;27(3):643-50. doi: 10.1046/j.1365-2958.1998.00714.x.


Amino acid transporters of the yeast plasma membrane (permeases) belong to a family of integral membrane proteins with pronounced structural similarity. We present evidence that a member of this family, encoded by the open reading frame (ORF) YDR160w (SSY1), is required for the expression of a set of transporter genes. Thus, deletion of the SSY1 gene causes loss of leucine-inducible transcription of the amino acid permease genes BAP2, TAT1 and BAP3 (ORF YDR046c) and the peptide transporter, PTR2. D-leucine can generate the signal without entering the cell. We propose that Ssy1p is situated in the plasma membrane and is involved in sensing leucine in the medium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Transport Systems
  • Amino Acids / metabolism
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Fungal Proteins / metabolism
  • Gene Deletion
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal
  • Leucine / metabolism
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Membrane Transport Proteins / genetics*
  • Membrane Transport Proteins / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Signal Transduction
  • Transcription, Genetic
  • Transcriptional Activation


  • Amino Acid Transport Systems
  • Amino Acids
  • Carrier Proteins
  • Fungal Proteins
  • Membrane Proteins
  • Membrane Transport Proteins
  • PTR2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Leucine