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, 422 (2), 259-64

The First Constant Domain (C(H)1 and C(L)) of an Antibody Used as Heterodimerization Domain for Bispecific Miniantibodies

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The First Constant Domain (C(H)1 and C(L)) of an Antibody Used as Heterodimerization Domain for Bispecific Miniantibodies

K M Müller et al. FEBS Lett.

Abstract

Bispecific miniantibodies were constructed by genetically fusing the C(H)1 domain of an IgG1 to the C-terminus of a single-chain Fv fragment (scFv-425), specific for the EGF receptor, and fusing the C(L) domain of a kappa light chain to the C-terminus of a scFv specific for CD2 (scFv-M1). An efficient dicistronic gene arrangement for functional expression in Escherichia coli was constructed. Immunoblots demonstrated correct domain assembly and the formation of the natural C(H)1-C(L) disulfide bridge. Gel filtration confirmed the correct size, sandwich ELISAs demonstrated bispecific functionality, and SPR biosensor measurements determined binding to EGF-R in comparison to bivalent constructs. Bispecific anti-EGF-R/anti-CD2 miniantibodies are candidates for the immunotherapy of cancer.

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