Molecular cloning, genomic characterization and expression of novel human alpha1A-adrenoceptor isoforms

FEBS Lett. 1998 Jan 30;422(2):279-83. doi: 10.1016/s0014-5793(98)00024-6.


We have isolated and characterized from human prostate novel splice variants of the human alpha1A-adrenoceptor, several of which generate truncated products and one isoform, alpha(1A-4), which has the identical splice site as the three previously described isoforms. Long-PCR on human genomic DNA showed that the alpha(1A-4) exon is located between those encoding the alpha(1A-1) and alpha(1A-3) variants. CHO-K1 cells stably expressing alpha(1A-4) showed ligand binding properties similar to those of the other functional isoforms as well as agonist-stimulated inositol phosphate accumulation. Quantitative PCR analyses revealed that alpha(1A-4) is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.

MeSH terms

  • Adrenergic alpha-Antagonists / pharmacology*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CHO Cells
  • Cloning, Molecular
  • Cricetinae
  • Gene Library
  • Humans
  • Inositol Phosphates / metabolism*
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Prostate / metabolism*
  • RNA, Messenger / metabolism
  • Radioligand Assay
  • Receptors, Adrenergic, alpha-1 / biosynthesis
  • Receptors, Adrenergic, alpha-1 / chemistry
  • Receptors, Adrenergic, alpha-1 / physiology*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transfection


  • ADRA1A protein, human
  • Adrenergic alpha-Antagonists
  • Inositol Phosphates
  • RNA, Messenger
  • Receptors, Adrenergic, alpha-1
  • Recombinant Proteins

Associated data

  • GENBANK/AF013261