Identification of the lamina-associated-polypeptide-2-binding domain of B-type lamin

Eur J Biochem. 1998 Feb 1;251(3):729-33. doi: 10.1046/j.1432-1327.1998.2510729.x.

Abstract

Lamina-associated polypeptide (LAP)2, which directly interacts with B-type lamins and chromosomes, is an integral membrane protein specifically distributed along the inner nuclear membrane of the nuclear envelope. Multiple regions of its large nucleoplasmic domain promote this localization, including the first (residues 1-296) and the second (residues 298-409) halves of the LAP2 N terminus. The second half is involved in LAP2 association with the nuclear lamina [Furukawa, K., Panté, N., Aebi, U. & Gerace, L. (1995) EMBO J. 14, 1626-1636]. In this study to further define its role, we examined which domain of B-type lamin interacts with LAP2 by means of a binding assay with bacterially expressed proteins and a yeast two-hybrid system. We found that amino acids in the region of residues 78-258 of the lamin B1 rod domain directly bound with LAP2. The data suggest that LAP2 may modulate the assembly of nuclear lamins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Cell Nucleus / metabolism
  • Chromosomes / metabolism
  • Cloning, Molecular
  • DNA-Binding Proteins*
  • Lamin Type B*
  • Lamins
  • Membrane Proteins / chemistry*
  • Membrane Proteins / metabolism*
  • Mutagenesis, Site-Directed
  • Nuclear Proteins / biosynthesis
  • Nuclear Proteins / chemistry*
  • Nuclear Proteins / metabolism*
  • Polymerase Chain Reaction
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae
  • Sequence Deletion

Substances

  • DNA-Binding Proteins
  • Lamin Type B
  • Lamins
  • Membrane Proteins
  • Nuclear Proteins
  • Recombinant Proteins
  • lamin B1
  • lamina-associated polypeptide 2