Directed termination PCR: a one-step approach to mutation detection

Nucleic Acids Res. 1998 Mar 15;26(6):1546-7. doi: 10.1093/nar/26.6.1546.


We describe a novel PCR-based method that allows the generation of nested termination fragments by integrating both selective DNA amplification and directed chain termination into a single PCR reaction. These termination fragments can be examined for sequence variation in either denaturing or non-denaturing polyacrylamide gels. This method provides a one-step and highly effective approach for the detection of both insertions/deletions and single base pair substitutions in sequences up to 1 kb in length.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins
  • Animals
  • DNA Mutational Analysis / methods*
  • DNA, Mitochondrial / genetics
  • DNA, Mitochondrial / isolation & purification
  • Genes, BRCA1
  • Humans
  • Ictaluridae / genetics
  • Infant, Newborn
  • Mutation*
  • Nucleic Acid Denaturation
  • Point Mutation
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Single-Stranded Conformational
  • Sequence Deletion


  • Acrylic Resins
  • DNA, Mitochondrial
  • polyacrylamide gels